Coding

Part:BBa_K2980010:Design

Designed by: Ji Gao   Group: iGEM19_Tsinghua   (2019-09-19)


Cry2-mCherry-xylE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 497
    Illegal BglII site found at 956
    Illegal BamHI site found at 1435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2767
    Illegal NgoMIV site found at 2939
    Illegal AgeI site found at 381
    Illegal AgeI site found at 1110
    Illegal AgeI site found at 3290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 733
    Illegal BsaI.rc site found at 142
    Illegal SapI.rc site found at 250


Design Notes

Constructed by Hifi assembly.


Source

Please refer to each Basic parts.

References